Full-Time Post-doc Immunology
As a post-doc, you will work within the Department of Infectious Diseases, group ‘immunology and immunogenetics of Mycobacterial infectious diseases’, on the project ‘In depth characterization of Mycobacterium tuberculosis (Mtb) specific HLA-E restricted human CD8+ T-cells’. The project’s research involves advanced cellular immunology in combination with molecular biology.
The research project contributes to the development of vaccines against tuberculosis (TB). An important job if you realize that approximately one-third of the world population is latently infected with Mycobacterium tuberculosis (Mtb), and that every infected person has a lifetime chance of 10% to develop active TB disease. It has been estimated that between 2007 and 2027 approximately 1 billion people will be infected with Mtb, 150 million people will develop TB disease and 36 million people will die of TB disease. We will only be able to develop better vaccines and antibiotics against TB if we have a better understanding of the immune response towards Mtb.
T-cell immunity is an important component of the immune response towards Mtb. Infection with Mtb results in activation of multiple different T-cell subsets, including classical Th1 subsets, but also non-classically restricted CD8+ T-cells. We are in particularly interested in CD8+ T-cells that recognize Mtb peptides in the context of the low polymorphic HLA-E molecule, potentially a very interesting vaccination target. Surprisingly, human CD8+ T-cells recognizing Mtb peptides in HLA-E produce Th2 cytokines and possess the capacity to control intracellular Mtb outgrowth. In the present project we aim to characterize these T-cells in great detail using advanced flowcytometry, cell sorting and RNA sequencing.
The Department of Infectious Diseases of the Leiden University Medical Center (LUMC) is a renowned centre of excellence. It provides clinical inpatient and outpatient care, modern education, and innovative basic, translational and clinical research in the area of infectious diseases, pursuing excellence in each of these areas.
We seek an enthusiastic and dedicated candidate with a completed PhD degree. Experience in cellular immunology, including (primary) cell culture and flowcytometry (FACS Canto/LSRII/ LSR-Fortessa) is essential, additional experience in molecular biology and analysis of large data sets would be highly valuable. Candidates are expected to have experience with basic immunological techniques such as ELISA, multiplex bead arrays (Luminex) and sterile cell culture (BSLI/II). Moreover, the candidate has the ability to work independently within a dynamic team and outstanding skills in written and oral communication in english.